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Chemistry Department Nuclear Magnetic Resonance Spectroscopy (NMR) Facility |
Sample preparation
For typical Proton experiments use 10-20 mg/ml of sample and for typical Carbon experiments use 50-100mg/ml of sample. (Some Carbon experiments would require higher concentrations or longer acquisition times). Choose a solvent that meets your needs, as well as the needs of the planned experiment. If you have a liquid sample, prepare a 10% v/v solution (make sure that the solvent contains an NMR standard). Pipette approx. 0.75ml of the solution into the NMR tube.
It is important that the quality of your tubes is minimum or better from what is required for specific magnetic field. The better quality would result in better quality spectra. Use of low quality, disposable or damaged NMR tubes is not allowed because it can cause serious damage to the probe and will alter the measurement. Make sure that your tube is properly capped and that the outside walls are clean before placing it into a magnet. (Take special care while handling the tube if your sample is toxic).